DNA replicationOptical cross section through a mouse epithelial cell nucleus imaged with 3D-SIM. The DNA replication machinery (green) is immunolabelled with an anti-PCNA antibody, post replicative DNA (red) is stained by EdU pulse labelling (red), and chromatin (blue) is stained with DAPI. The comparison with the conventional wide-field image (upper left) illustrates the increased level of details obtained with 3D-SIM.
Mouse myoblast cell nucleus in early stage of cell divisionimaged with super-resolution 3D-structured illumination microscopy. Condensing chromosomes are stained with DAPI (red). The nuclear lamina is immunolabelled with an anti-lamin B antibody (green). 3D ray tracing rendering gives an impression of the irregular meshwork of filaments constituting the nuclear envelope. The two depressions at the top side indicate the centrosome positions. The characteristic infoldings of the lamina are induced by the formation of the mitotic spindle. Initial tearing of the lamina can be seen in the lower part of the nucleus. (Schermelleh et al. 2008, Science 320)
HeLa cell in mitosis imaged with super-resolution 3D-structured illumination microscopy. Microtubules forming the mitotic spindle (green) are immunolabelled with anti-tublin antibodies. Condensed chromosomes (red) are stained with DAPI. Projection view of a 3D image stack (Dunsch et al.,2013, J Cell Biol198).