Peer-reviewed Articles & Chapters (Pubmed listed)

(#co-senior authors; *co-first authors)

2017

(56) Demmerle J, Innocent C, North AJ, Ball G, Müller M, Miron E, Matsuda A, Dobbie IM, Markaki Y, Schermelleh L. Strategic and practical guidelines for successful structured illumination microscopy. Nat Protoc. 12(5):988-1010. DOI: 10.1038/nprot.2017.019.

(55) Kraus F, Miron E, Demmerle J, Budco A, Chitiashvili T, Alle Q, Matsuda A, Leonhardt H, Schermelleh L#, Markaki Y#. Quantitative 3D structured illumination microscopy of nuclear structures. Nat Protoc. 12(5):1011-1028. DOI: 10.1038/nprot.2017.020.

2016

(54) Miron E, Innocent C, Heyde S, Schermelleh L. 2016, In Vivo and In Situ Replication Labeling Methods for Super-resolution Structured Illumination Microscopy of Chromosome Territories and Chromatin Domains. Methods Mol Biol. 2016;1431:127-40. DOI: 10.1007/978-1-4939-3631-1_10.

(53) Chagin VO, Casas-Delucchi CS, Reinhart M, Schermelleh L, Markaki Y, Maiser A, Bolius JJ, Bensimon A, Fillies M, Domaing P, Rozanov YM, Leonhardt H, Cardoso MC. 2016. 4D Visualization of replication foci in mammalian cells corresponding to individual replicons. Nat Commun. 7:11231. DOI: 10.1038/ncomms11231.

2015

(52) Hagen C, Dent KC, Zeev-Ben-Mordehai T, Grange M, Bosse JB, Whittle C, Klupp BG, Siebert AC, Vasishtan D, Baeuerlein FJB,  Cheleski J, Werner S, Guttmann P, Rehbein S, Henzler K, Demmerle J, Adler B,  Koszinowski U, Schermelleh L,  Schneider G, Enquist LW, Plitzko JM,  Mettenleiter TC, Gruenewald K. 2015. Structural Basis of Vesicle Formation at the Inner Nuclear Membrane. Cell. 163: 1692-1701. DOI: 10.1016/j.cell.2015.11.029.

(51) Ball G, Demmerle J, Kaufmann R, Davis I, Dobbie IM, Schermelleh L. 2015. SIMcheck: a toolbox for successful super-resolution SIM imaging. 2015. Sci Rep. 5: 15915. doi: 10.1038/srep15915. Erratum in Sci Rep 2016; 6:20754. DOI: 10.1038/srep20754.

(50) Moindrot B, Cerase A, Coker H, Masui O, Grijzenhout A, Pintacuda G, Schermelleh L, Nesterova TB, Brockdorff N. 2015. A pooled shRNA screen identifies Rbm15, Spen and Wtap as factors required for Xist RNA mediated silencing. Cell Reports. 12: 562-72. DOI: 10.1016/j.celrep.2015.06.053.

(49) Demmerle J, Wegel E, Schermelleh L, Dobbie IM. 2015. Assessing resolution in super-resolution imaging. Methods. 88: 3-10. DOI: 10.1016/j.ymeth.2015.07.001.

2014

(48) Conduit PT, Richens JH, Wainman A, Holder J, Vicente CC, Pratt MB, Dix CI, Novak ZA, Dobbie IM, Schermelleh L, Raff JW. 2014. A molecular mechanism of mitotic centrosome assembly in Drosophila. eLife. 3: e03399. DOI: 10.7554/eLife.03399.

(47) Smeets D, Markaki Y, Schmid VJ, Kraus F, Tattermusch A, Cerase A, Sterr M, Popken J, Leonhardt H, Brockdorff N, Cremer T#, Schermelleh L#, Cremer M#. 2014. 3D super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci. Epigenetics Chromatin. 7: 8. DOI: 10.1186/1756-8935-7-8.

(46) Cooper S, Dienstbier M, Hassan R, Schermelleh L, Sharif J, Blackledge NP, De Marco V, Elderkin S, Koseki H, Klose R, Heger A, Brockdorff N. 2014. Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment. Cell Reports. 7: 1456-70. DOI: 10.1016/j.celrep.2014.04.012.

(45) Osseforth C, Moffitt JR, Schermelleh L, Michaelis J. 2014. Simultaneous dual-color 3D STED microscopy. Opt Express. 22: 7028-39. DOI: 10.1364/OE.22.007028.

(44) Cerase A, Smeets D, Tang AY, Gdula M, Kraus F, Spivakov M, Moindrot B, Leleu M, Tattermusch A, Demmerle J, Nesterova T, Green C, Otte AP, Schermelleh L, Brockdorff N. 2014. Spatial separation of Xist RNA and polycomb proteins revealed by super-resolution microscopy. Proc Natl Acad Sci USA. 111: 2235-40. DOI: 10.1073/pnas.1312951111.

(43) Lesterlin C, Ball G, Schermelleh L, Sherratt D. 2014. DRecA bundles mediate homology pairing between distant sisters during DNA break repair. Nature. 506: 249-53. DOI: 10.1038/nature12868. Epub 2013 Dec 22.

2013

(41) Wolf A, Mantri M, Heim A, Müller U, Fichter E, Mackeen MM, Schermelleh L, Dadie G, Leonhardt H, Vénien-Bryan C, Kessler BM, Schofield CJ, Böttger A. 2013. The polyserine domain of the lysyl-5 hydroxylase Jmjd6 mediates subnuclear localization. Biochem J. 453: 357-70. DOI: 10.1042/BJ20130529.

(40) Bergink S, Ammon T, Kern M, Schermelleh L, Leonhardt H, Jentsch S. 2013. Role of Cdc48 (p97) as a SUMO-targeted segregase curbing Rad51-Rad52 interaction. Nat Cell Biol. 15: 526-32. DOI: 10.1038/ncb2729.

(39) Hahn M, Dambacher S, Dulev S, Kuznetsova AY, Eck S, Wörz S, Sadic D, Schulte M, Mallm J, Maiser A, Debs P, von Melchner H, Leonhardt H, Schermelleh L, Rohr K, Rippe K, Storchova Z, Schotta G. 2013. Suv4-20h2 mediates chromatin compaction and is important for cohesin recruitment to heterochromatin. Genes Dev. 27: 859-72. DOI: 10.1101/gad.210377.112.

(38) Schneider K*, Fuchs C*, Dobay A, Rottach A, Qin W, Álvarez-Castro JM, Nalaskowski MM, Schmid V, Leonhardt H, Schermelleh L. 2013. Dissection of cell cycle dependent dynamics of Dnmt1 by FRAP and diffusion-coupled modelling. Nucleic Acids Res. 41: 4860-76. DOI: 10.1093/nar/gkt191.

(37) Markaki Y, Smeets D, Cremer M, Schermelleh L. 2013. Fluorescence in situ hybridization applications for super-resolution 3D structured illumination microscopy. Methods Cell Biol. 950: 43-64. DOI: 10.1007/978-1-62703-137-0_4.

2012

(36) Dunsch AK, Hammond D, Lloyd J, Schermelleh L, Gruneberg U, Barr FA. 2012. Asymmetric cortical localisation of dynein during spindle orientation requires dynein light chain 1 and a spindle-associated adaptor complex. J Cell Biol. 198: 1039-54. DOI: 10.1083/jcb.201202112.

(35) Sonnen KF, Schermelleh L, Leonhardt H, Nigg EA. 2012. 3D-structured illumination microscopy provides novel insight into architecture of human centrosomes. Biology Open. 1: 965-76. DOI: 10.1242/bio.20122337.

(34) Hochstatter J, Holzel M, Rohrmoser M, Schermelleh L, Leonhardt H, Keough R, Gonda TJ, Imhof A, Eick D, Langst G, Nemeth A. 2012. Myb-binding protein 1a (Mybbp1a) regulates levels and processing of Pre-ribosomal RNA. J Biol Chem. 287: 24365-77. DOI: 10.1074/jbc.M111.303719.

(33) Markaki Y, Smeets D, Fiedler S, Schmid VJ, Schermelleh L, Cremer T, Cremer M. 2012. The potential of 3D-FISH and super-resolution structured illumination microscopy for studies of 3D nuclear architecture. Bioessays. 34: 412-26. DOI: 10.1002/bies.201100176.

(32) Mikeladze-Dvali T, von Tobel L, Strnad P, Knott G, Leonhardt H, Schermelleh L, Gönczy P. 2012. Analysis of centriole elimination during C. elegans oogenesis. 2012. Development. 139: 1670-9. DOI: 10.1242/dev.075440.

(31) Bönisch C, Schneider K, Pünzeler S, Wiedemann SM, Bielmeier C, Bocola M, Eberl HC, Kuegel W, Neumann J, Kremmer E, Leonhardt H, Mann M, Michaelis J, Schermelleh L#, Hake SB#. 2012. H2A.Z.2.2 is an alternatively spliced histone H2A.Z variant that causes severe nucleosome destabilization. Nucleic Acids Res. 40: 5951-64. DOI: 10.1093/nar/gks267.

(30) Ratnakumar K, Duarte LF, LeRoy G, Hasson D, Smeets D, Vardabasso C, Bönisch C, Zeng T, Xiang B, Zhang DY, Li H, Wang X, Hake SB, Schermelleh L, Garcia BA, Bernstein E. 2012. ATRX-mediated chromatin association of histone variant macroH2A1 regulates alpha globin expression. Genes Dev. 26: 433-8. DOI: 10.1101/gad.179416.111.

(29) Lebold T, Schlossbauer A, Schneider K, Schermelleh L, Leonhardt H, Bein T, Bräuchle T. 2012. Controlling the mobility of oligonucleotides in the nanochannels of mesoporous silica. Adv Funct Mat. 22: 106-12. DOI: 10.1002/adfm.201101365 (peer-reviewed, not Pubmed listed).

2011

(27) Markaki Y, Gunkel M, Schermelleh L, Beichmanis S, Neumann J, Heidemann M, Leonhardt H, Eick D, Cremer C, Cremer T. 2011. Functional nuclear organization of transcription and DNA replication: a topographical marriage between chromatin domains and the interchromatin compartment Cold Spring Harb Symp Quant Biol. 75: 475-92. DOI: 10.1101/sqb.2010.75.042.

(26) Cordes T, Maiser A, Steinhauer C, Schermelleh L#, Tinnefeld P#. 2011. Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy. Phys Chem Chem Phys. 13: 6699-709. DOI: 10.1039/c0cp01919d.

(25) Guizetti J, Schermelleh L, Mäntler J, Maar S, Poser I, Leonhardt H, Müller-Reichert T, Gerlich DW. 2011. Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments. Science. 331: 1616-20. doi: 10.1126/science.1201847.

(24) Larochelle N, Stucka R, Rieger N, Schermelleh L, Schiedner G, Kochanek S, Wolf E, Lochmüller. 2011. Genomic integration of adenoviral gene transfer vectors following transduction of fertilized mouse oocytes. Transgenic Res. 20: 123-35. doi: 10.1007/s11248-010-9401-x. Epub 2010 May 13.

2010

(23) Wiedemann SM, Mildner SN, Bönisch C, Israel L, Maiser A, Matheisl S, Straub T, Merkl R, Leonhardt H, Kremmer E, Schermelleh L, Hake SB. 2010. Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. J Cell Biol. 190: 777-91. DOI: 10.1083/jcb.201002043.

(22) Schermelleh L#, Heintzmann R#, Leonhardt H#. 2010. A guide to super-resolution fluorescence microscopy. J Cell Biol. 190: 165-75. DOI: 10.1083/jcb.201002018. Review.

(21) Baddeley D, Chagin VO, Schermelleh L, Martin S, Pombo A, Carlton PM, Gahl A, Domaing P, Birk U, Leonhardt H, Cremer C, Cardoso MC. 2010. Measurement of replication structures at the nanometer scale using super-resolution light microscopy. Nucleic Acids Res. 38: e8. DOI: 10.1093/nar/gkp901. Epub 2009 Oct 28.

2008

(20) Schermelleh L, Spada F, Leonhardt H. 2008. Visualization and Measurement of DNA Methyltransferase Activity in Living Cells. Curr Protoc in Cell Biol. Chapter 22: Unit 22 12. DOI: 10.1002/0471143030.cb2212s39.

(19) Schermelleh L*, Carlton PM*, Haase S, Shao L, Winoto L, Kner P, Burke B, Cardoso MC, Agard DA, Gustafsson MG, Leonhardt H, Sedat JW. 2008. Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Science. 320: 1332-6. DOI: 10.1126/science.1156947.

(18) Kuch D, Schermelleh L, Manetto S, Leonhardt H, Carell T. 2008. Synthesis of DNA dumbbell based inhibitors for the human DNA methyltransferase Dnmt1. Angew Chem Int Ed Engl. 47: 1515-8. DOI: 10.1002/anie.200702055.

2007

(17) Schermelleh L, Haemmer A, Spada F, Rösing N, Meilinger D, Rothbauer U, Cardoso MC, Leonhardt H. 2007. Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation. Nucleic Acids Res. 35: 4301-12. DOI: 10.1093/nar/gkm432.

(16) Spada F, Haemmer A, Kuch D, Rothbauer U, Schermelleh L, Kremmer E, Carell T, Längst G, Leonhardt H. 2007. DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells. J Cell Biol. 176: 565-71. DOI: 10.1083/jcb.200610062.

2006

(15) Albiez H, Cremer M, Tiberi C, Vecchio L, Schermelleh L, Dittrich S, Küpper K, Joffe B, Thormeyer T, von Hase J, Yang S, Rohr K, Leonhardt H, Solovei I, Cremer C, Fakan S, Cremer T. 2006. Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks. Chromosome Res. 14: 707-33. DOI: 10.1007/s10577-006-1086-x.

(14) Rothbauer U, Zolghadr K, Tillib S, Nowak D, Schermelleh L, Gahl A, Backmann N, Conrath K, Muyldermans S, Cardoso MC, Leonhardt H. 2006. Targeting and tracing antigens in live cells with fluorescent nanobodies. Nat Methods. 3: 887-9. DOI: 10.1038/nmeth953.

(13) Spada F, Rothbauer U, Zolghadr K, Schermelleh L, Leonhardt H. 2006. Regulation of DNA methyltransferase 1. Adv. Enzym Regul. 46: 224-34. DOI: 10.1016/j.advenzreg.2006.01.011. Review.

 

2005

(10) Schermelleh L, Spada F, Easwaran HP, Zolghadr K, Margot JB, Cardoso MC, Leonhardt H. 2005. Trapped in action: direct visualization of DNA methyltransferase activity in living cells. Nat Methods. 2: 751-6. DOI: 10.1038/nmeth794.

(9)   Grohmann M, Spada F, Schermelleh L, Alenina N, Bader M, Cardoso MC, Leonhardt H. 2005. Restricted mobility of Dnmt1 in preimplantation embryos: implications for epigenetic reprogramming. BMC Dev Biol. 5: 18. DOI: 10.1186/1471-213X-5-18.

(8)   Mortusewicz O, Schermelleh L, Walter J, Cardoso MC, Leonhardt H. 2005. Recruitment of DNA methyltransferase I to DNA repair sites. Proc Natl Acad Sci USA. 102: 8905-9. DOI: 10.1073/pnas.0501034102.

 

2004

(7)   Easwaran HP, Schermelleh L, Leonhardt H, Cardoso MC. 2004. Replication-independent chromatin loading of Dnmt1 during G2 and M phases. EMBO Rep. 5: 1181-6. DOI: 10.1038/sj.embor.7400295.

(6)   Solovei I, Schermelleh L, Düring K, Engelhardt A, Stein S, Cremer C, Cremer T. 2004. Differences in centromere positioning of cycling and postmitotic human cell types. Chromosoma. 112: 410-23. DOI: 10.1007/s00412-004-0287-3.

 

1999-2003

(5)   Walter J*, Schermelleh L*, Cremer M, Tashiro S, Cremer T. 2003. Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages. J Cell Biol. 160: 685-97. DOI: 10.1083/jcb.200211103.

(4)   Solovei I, Cavallo A, Schermelleh L, Jaunin F, Scasselati C, Cmarko D, Cremer C, Fakan S, Cremer T. 2002. Spatial Preservation of Nuclear Chromatin Architecture during Three-Dimensional Fluorescence in Situ Hybridization (3D-FISH). Exp Cell Res. 276: 10-23. DOI:10.1006/excr.2002.5513.

(2)   Schermelleh L, Solovei I, Zink D, Cremer T. 2001. Two-color fluorescence labeling of early and mid-to-late replicating chromatin in living cells. Chromosome Res. 9: 77-80.

(1)   Schermelleh L, Thalhammer S, Heckl W, Pösl H, Cremer T, Schütze K, Cremer M. 1999. Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes. Biotechniques. 27: 362-7.

 

Book Chapters

 (42) Smeets D, Neumann J, Schermelleh L. 2014. Application of three-dimensional structured illumination microscopy in cell biology – pitfalls and practical considerations. In Super-Resolution Microscopy Techniques in the Neurosciences, Neuromethods Vol. 86, Fornasiero E, Rizzoli S (Eds.) Springer. 167-88. DOI: 10.1007/978-1-62703-983-3_8.

(28) Cremer T, Markaki Y, Hübner B, Zunhammer A, Strickfaden H, Beichmanis S, Heß M, Schermelleh L, Cremer M, Cremer C. 2012. Chromosome territory organization within the nucleus. In Epigenetic Regulation and Epigenomics: Advances in Molecular Biology and Medicine 1st edition, Meyers RE (Ed.). Wiley. 451-83.  

(12) Schermelleh L. 2005. In vivo replication labeling. In Cell Biology: A Laboratory Handbook 3rd edition, Celis JE (Ed.). Elsevier Science. 301-3.

(11) Solovei I, Schermelleh L, Albiez H, Cremer T. 2005. Detection of the cell cycle stages in situ in growing cell populations. In Cell Biology: A Laboratory Handbook 3rd edition, Celis JE (Ed.). Elsevier Science. 291-9.

(3)   Solovei I, Walter J, Cremer M, Habermann F, Schermelleh L, Cremer T. 2002. FISH on three-dimensionally preserved nuclei. In FISH: a practical approach. Squire J, Beatty B, Mai S (Eds.) Oxford University Press, Oxford. 119-57.